mouse tissue Search Results


99
R&D Systems anti mtf antibody
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R&D Systems tissue microarray
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Miltenyi Biotec adipose tissue dissociation kit
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R&D Systems anti tissue factor cd142
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Novus Biologicals mouse heart tissue lysate
FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat <t>tissue</t> extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell <t>lysate</t> (TLR10) and <t>mouse</t> <t>heart</t> whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.
Mouse Heart Tissue Lysate, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec mitochondria isolation kit mouse tissue
FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat <t>tissue</t> extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell <t>lysate</t> (TLR10) and <t>mouse</t> <t>heart</t> whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.
Mitochondria Isolation Kit Mouse Tissue, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
R&D Systems mouse brain tissue
FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat <t>tissue</t> extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell <t>lysate</t> (TLR10) and <t>mouse</t> <t>heart</t> whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.
Mouse Brain Tissue, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

Journal: Biology of reproduction

Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

doi: 10.1095/biolreprod.106.059410

Figure Lengend Snippet: FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

Article Snippet: Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.

Techniques: Western Blot, Control, Positive Control, Transformation Assay